This two-volume set constitutes the refereed proceedings of the 16th Asian Conference on Intelligent Information and Database Systems, ACIIDS 2024, held in Ras Al Khaimah, UAE, during April 15-18, 2024. The 57 full papers presented in these proceedings were carefully reviewed and selected from 251 submissions. The papers of the 2 volume-set are organized in the following topical sections:  Part I: advanced data analysis methods; AI-driven cybersecurity and medical solutions; application of generative models; computational intelligence. Part II: data modelling and smart information systems; image and video processing; machine learning algorithms in theory and practice; prediction and recommendation systems; text, speech and natural language processing.