This book collects papers from the 20 ISATT International Biennial Conference held at the ‘Aldo Moro’ University of Bari in 2023. The central theme is hybridization in teaching and teacher education, reflected in the book’s title. Its subtitle highlights innovative practices and methodological challenges from theoretical and empirical perspectives. Divided into three sections—frameworks, methodological investigations, and practical cases—the book presents contributions from researchers worldwide, offering insights and evidence on the topic, fostering scholarly networks, and advancing discussions on hybrid teaching and teacher training contexts.