This fully updated edition explores current techniques for research into antibiotic resistance. The book begins with how samples are collected, strains isolated and sequenced, and the results integrated in the microbiological workflow.  It continues with novel methods to test resistance and interactions between antibiotics, physiological conditions, or using innovative tools like the hollow fiber or Raman spectroscopy, as well as mathematical models that can describe resistance within host. Written for the highly successful  Methods in Molecular Biology  series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date,  Antibiotic Resistance Protocols, Fourth Edition  serves as an ideal guide for answering questions on how to control antibiotic resistance, to develop new agents, and to address the problems posed by microbes that have become resistant to our antibiotics.