This book constitutes revised selected papers from the 16th International Symposium on Algorithms and Experiments for Wireless Sensor Networks, ALGOSENSORS 2020, held in Pisa, Italy*, in September 2020. The 12 full papers presented in this volume were carefully reviewed and selected from 27 submissions. ALGOSENSORS is an international symposium dedicated to the algorithmic aspects of wireless networks. *The conference was held virtually due to the COVID-19 pandemic.