This book gathers the proceedings of the 5th International Conference on Nanotechnologies and Biomedical Engineering, held online on November 3-5, 2021, from Chisinau, Republic of Moldova. It covers fundamental and applied research at the interface between nanotechnologies and biomedical engineering. Chapters report on cutting-edge bio-micro/nanotechnologies, devices for biomedical applications, and advances in bio-imaging and biomedical signal processing, innovative nano-biomaterials as well as advances in e-health, medical robotics, and related topics. With a good balance of theory and practice, the book offers a timely snapshot of multidisciplinary research at the interface between physics, chemistry, biomedicine, materials science, and engineering.